Aldo-Keto Reductases Protect Lung Adenocarcinoma Cells from the Acute Toxicity of B[a]P-7,8-trans-Dihydrodiol

Tobacco smoke exposure stimulates the expression of genes that are likely to be involved in the metabolism of its combustion products such as polycyclic aromatic hydrocarbons (PAH). These data suggest that over expression of aldo-keto reductases may protect lung cancer cells from the acute toxic effects of PAH. Four of the smoke induced genes are aldo-keto reductases (AKR), enzymes that metabolically activate PAH to PAH o-quinones. Alternatively, PAHs are metabolised to anti-diol epoxides, such as antibenzo a]pyrene diol epoxides -anti-BPDE, by the combined action of P 4501A1/1B1 and epoxide hydrolase. Anti-BPDE forms DNA adducts directly, while PAH o-quinones cause DNA damage by oxidative stress through a futile redox cycle. To address the role of AKRs in PAH cytotoxicity, the authors compared the cytotoxicity of PAH metabolites and the effects of over expressing AKR1A1 in lung cells. Anti-BPDE and B[fusion_builder_container hundred_percent=”yes” overflow=”visible”][fusion_builder_row][fusion_builder_column type=”1_1″ background_position=”left top” background_color=”” border_size=”” border_color=”” border_style=”solid” spacing=”yes” background_image=”” background_repeat=”no-repeat” padding=”” margin_top=”0px” margin_bottom=”0px” class=”” id=”” animation_type=”” animation_speed=”0.3″ animation_direction=”left” hide_on_mobile=”no” center_content=”no” min_height=”none”][a]P-7,8-trans-dihydrodiol, an intermediate in anti-BPDE metabolism, are toxic to A549 cells at concentrations with an IC50 of ~2 íM. In contrast, the PAH o-quinone B[a]P-7,8-dione was about 10-fold less toxic to A549 cells with an IC50 >20 1M. Similar differences in cytotoxicity were observed with two other PAH o-quinones (benz[a]anthracene-3,4-dione and 7,12-dimethylbenz[a]anthracene-3,4-dione) compared with their respective diol-epoxide counterparts (BA-3,4-diol-1,2-epoxide and DMBA-3,4-diol-1,2-epoxide). In addition both anti-BPDE and B[a]P-7,8-transdihydrodiol induced p53 expression 6 h post-treatment at concentrations as low as 1M consistent with extensive DNA damage. B[a]P-7,8-dione treatment did not induce p53 but generated reactive oxygen species (ROS) in A549 cells and induced the expression of oxidative response genes in H358 cells. The authors also observed that over expression of AKR1A1 in H358 cells, which otherwise have low levels of AKR expression, protected cells 2-10-fold from the toxic effects of B[a]P-7,8-trans-dihydrodiol.

Authors: Abedin, Zahidur; Sen, Sushmita; Field, Jeffrey ;Full Source: Chemical Research in Toxicology [online computer file] 2012, 25(1), 113-121 (Eng) ;