Assessment of toxicity of chemicals in workers of rubber industry

Various chemicals like polycyclic aromatic hydrocarbons [PAH], N-nitrosamines, 1, 3-butadiene, acetonitrile, styrene, vinyl chloride, mineral oils, other volatile organic compounds, etc. are generally used in manufacturing of rubber and have shown hazardous effects in human upon regular exposure. In view of the above, a study was carried out to determine variation in Hb%, TLC and differential leukocyte count, level of oxidative stress by estimating GSH concentration, genotoxic risk by studying micronuclei in lymphocytes and effect of compounds on various organs by studying the level of expression of LDH isoenzymes. The blood samples were collected from 20 rubber industry workers for each group and 20 healthy workers who served as control. A decreased concentration of Hb% and TLC was observed in workers with the increase in time of exposure, albeit in normal range. The results demonstrated a significant decline in the neutrophil, eosinophil, lymphocyte and monocyte counts in workers who were exposed to 4-6 years. The decreased levels of GSH concentration signify induction of oxidative stress in workers. The presence of micronuclei was observed in workers who were exposed to 4-6 years and confirm the genotoxic risk in the workers of them. The increased level of LDH expression (LDH 2, LDH 3, LDH 4 and LDH 5) indicated the injured cells of different tissues. The authors concluded that based on the present findings, workers of rubber industry encounters injurious effect due to chemical compound exposure.

Authors: Singh, Ram; Kumar, Sachin; Sharma, Sunita ;Full Source: Journal of Pharmacy Research [online computer file] 2011, 4(9), 3229-3231 (Eng) ;