Zinc oxide nanoparticles (ZnO NPs) are one of the most widely used nanomaterials in consumer products and industrial applications. As a result of all these uses, this has raised concerns regarding their potential toxicity. The authors previously found that candidate markers of idiopathic pulmonary fibrosis and lung cancer were significantly up-regulated in rat bronchoalveolar lavage fluid (BALF) following exposure to ZnO NPs by using a liquid chromatography (LC)-based proteomic approach. To achieve comprehensive protein identification analysis, the two-dimensional gel electrophosis (2-DE)-based proteomic workflow was used to analyse the differences in BALF proteins from rats that had been exposed to a high dose of 35?nm ZnO NPs. A total of 31 differentially expressed protein spots were excised from the gels and analysed by nanoLC-tandem mass spectrometry (MS/MS). Gene ontology (GO) annotation of these proteins showed that most of the differentially expressed proteins were involved in response to stimulus and inflammatory response processes. Moreover, pulmonary surfactant-associated protein D and gelsolin, biomarkers of idiopathic pulmonary fibrosis, were significantly up-regulated in rat BALF after ZnO NPs exposure (2.42- and 2.84-fold, respectively). The results obtained from this present study could provide a complementary consequence with our previous study and contribute to a better understanding of the molecular mechanisms involved in ZnO NP-induced lung disorders.
Authors: Juang YM, Chien HJ, Yang CY, Yeh HC, Cheng TJ, Lai CC. ;Full Source: Mass Spectrometry (Tokyo). 2017;6(2): S0066. doi: 10.5702/mass spectrometry. S0066. Epub 2017 Mar 24. ;