Computational and experimental analysis of the glycophosphatidylinositol-anchored proteome of the human parasitic nematode Brugia malayi

2019-11-26

Further characterisation of essential systems in the parasitic filarial nematode Brugia malayi is needed to better understand its biology, its interaction with its hosts, and to identify critical components that can be exploited to develop novel treatments. The production of glycophosphatidylinositol-anchored proteins (GPI-APs) is essential for eukaryotic cellular and physiological function. In addition, GPI-APs perform many important roles for cells. In this study, the authors characterised the B. malayi GPI-anchored proteome using both computational and experimental approaches. The authors used bioinformatic strategies to show the presence or absence of B. malayi GPI-AP biosynthetic pathway genes and to compile a putative B. malayi GPI-AP proteome using available prediction programs. These were verified in silico analyses using proteomics to identify GPI-AP candidates prepared from the surface of intact worms and from membrane enriched extracts. The study represents the first description of the GPI-anchored proteome in B. malayi and lays the groundwork for further exploration of this essential protein modification as a target for novel anthelmintic therapeutic strategies.

Authors: Mersha FB, Cortes LK, Luck AN, McClung CM, Ruse CI, Taron CH, Foster JM.
; Full Source: PLoS One. 2019 Sep 12;14(9): e0216849. doi: 10.1371/journal.pone.0216849. eCollection 2019.