With the aim of establishing exposure levels for hospital personnel preparing and administering cytostatic drugs (CDs), the authors present an innovative screening method based on the use of the desorption electrospray ionisation(DESI) interface coupled with a hybrid quadrupole linear ion trap mass spectrometer. A rapid, simple, and sensitive procedure was developed for the simultaneous surface monitoring of cyclophosphamide, dacarbazine, methotrexate, vincristine, gemcitabine, and cytarabine. A PTFE-printed glass slide was used as a passive sampler, while hydrophobic centres of Swiffer cloths were judged extremely efficient as wipe samplers. After the sampling period, the CD collectors were directly processed with the DESI-MS system without any further treatment. MS/MS confirmatory analysis was conducted using selected reaction monitoring in the positive ion mode and detection limits were evaluated. Values were at the picograms per square millimetre levels on the passive collector and at the picograms per square centimetre levels for the wipe ones. Direct determination on solid-state samples combined with mass spectrometry selectivity provided a powerful tool so far unapplied to occupational hygiene. Figure DESI interface detail (spray emitter and MS inlet) with 3D spacefill structures of the six cytostatic drugs analysed.
Authors: Fabrizi, Giovanni; Fioretti, Marzia; Mainero Rocca, Lucia; Curini, Roberta ;Full Source: Analytical and Bioanalytical Chemistry 2011, Ahead of Print (English) ;