Detection of paraquat concentration in blood plasma by HPLC to diagnose and prevent paraquat poisoning

The title method comprises preparing blood plasma sample, adding internal standard water solution and protein precipitant acetonitrile, vortex-mixing, centrifuging to obtain supernatant, performing separation by using universal C18 liquid chromatography column and ion-pair reagent as acid mobile phase (3mmol/L sodium dodecyl sulfonate-0.2% trifluoroacetic acid-acetonitrile-water mixed solution), detecting with a diode array detector at detection wavelength of 250-260 nm to obtain peak areas of the internal standard and paraquat, and calculating to obtain paraquat concentration through least squares linear regression. The inventive method has the advantages of simple pre-treatment of plasma sample, sensitive and rapid detection, and high clinical application value, and can rapidly detect the toxic substance and its blood concentration.

Authors: Wang, Yong; Qiu, Xiangjun; Liu, Ling; Xu, Renai; Wang, Jiangang ;Full Source: Faming Zhuanli Shenqing CN 102,288,696 (Cl. G01N30/02), 21 Dec 2011, Appl. 10,186,707, 5 Jul 2011; 7pp. (Ch). ;