DNA damage assessment in construction painters’ lymphocytes by comet assay

Exposure to chemicals is inevitable for certain occupational groups, particularly in industrial countries. Some cytogenetic tests such as comet assay are effectively utilised to screen genotoxic effects of these chemicals in humans. The purpose of this study has been to assess the DNA damage in construction painters’ lymphocytes using the comet assay. Fourteen male construction painters with 1 year of required work experience were randomly selected from the list of Painters’ Union in Gonabad city, Iran. In addition, 14 healthy male non-painters were randomly selected as controls. The DNA damage degree was determined using a fluorescence microscope and CometScore software. The obtained data were analysed employing independent t-test using SPSS software v. 14 at the significant level of p < 0.05. The mean age was 35.28 years for all participants who were nonsmokers and nonalcoholics. No significant differences were found between the two groups in terms of age, weight, height, body mass index, and systolic and diastolic blood pressure. The DNA damage in the painter group was significantly greater than that of the control group. Tail length (µm) was 12.63 ± 6.54 versus 2.90 ± 0.84, tail DNA (%) was 4.33 ± 1.84 versus 1.29 ± 0.43, and tail moment (µm) was 1.85 ± 0.98 versus 0.14 ± 0.20 in painters' and control groups (p < 0.001), respectively. The authors concluded that the chemicals used in oil paint may increase the DNA damage in painters' blood lymphocytes. Authors: Kianmehr M, Amiri M, Ebrahimzadeh-Bideskan A, Hajavi J. ;Full Source: Toxicology & Industrial Health. 2015 Aug 28. pii: 0748233715597347. [fusion_builder_container hundred_percent="yes" overflow="visible"][fusion_builder_row][fusion_builder_column type="1_1" background_position="left top" background_color="" border_size="" border_color="" border_style="solid" spacing="yes" background_image="" background_repeat="no-repeat" padding="" margin_top="0px" margin_bottom="0px" class="" id="" animation_type="" animation_speed="0.3" animation_direction="left" hide_on_mobile="no" center_content="no" min_height="none"][Epub ahead of print] ;[/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]