Effects of organic chemicals from diesel exhaust particles on adipocytes differentiated from human mesenchymal stem cells

2022-10-10

Exposure to fine particulate matter (PM2.5 ) from incomplete fossil fuel combustion (coal, oil, gas and diesel) has been linked to increased morbidity and mortality due to metabolic diseases. PM2.5 exaggerate adipose inflammation and insulin resistance in mice with diet-induced obesity. Here we elucidate the hypothesis that such systemic effects may be triggered by adhered particle components affecting adipose tissue directly. Studying adipocytes differentiated from primary human mesenchymal stem cells, we found that lipophilic organic chemicals (OC) from diesel exhaust particles induced inflammation associated genes and increased secretion of the chemokine CXLC8/interleukin-8 as well as matrix metalloprotease 1. The oxidative stress response gene heme oxygenease-1 and tumour necrosis factor alpha were seemingly not affected, while aryl hydrocarbon receptor regulated genes, cytochrome P450 1A1 (CYP1A1) and CYP1B1 and plasminogen activator inhibitor-2, were clearly upregulated. Finally, expression of β-adrenergic receptor, known to regulate adipocyte homeostasis, was downregulated by exposure to these lipophilic OC. Our results indicate that low concentrations of OC from combustion particles have the potential to modify expression of genes in adipocytes which may be linked to metabolic disease. Further studies on mechanisms linking PM exposure and metabolic diseases are warranted.

Authors: Bendik C Brinchmann, Jørn A Holme, Nadine Frerker, Mia H Rambøl, Tommy Karlsen, Jan E Brinchmann, Alena Kubátová, Klara Kukowski, Tonje Skuland, Johan Øvrevik
; Full Source: Basic & clinical pharmacology & toxicology 2022 Oct 10. doi: 10.1111/bcpt.13805.