Evaluation of the effect of skin cleaning procedures on the dermal absorption of chemicals

To reduce the internal exposure, skin decontamination is the most important measure after dermal contact to chemicals. However, no harmonised skin cleaning procedure for experimental ex vivo studies is published. This study evaluated the impact of two skin cleaning techniques on dermal penetration kinetics and intradermal deposition of 1,4-dioxane, 5% hydrofluoric acid (HF, detected in terms of fluoride ions), and anisole to develop a reliable ex vivo skin cleaning method using the diffusion cell technique. After exposure (duration: 3min (HF); 1h (1,4-dioxane and anisole)) of excised human skin (n=6-8), decontamination was performed by (I) water-soaked cotton swabs or (II) direct application of water on the exposure area. The effect of skin cleaning was investigated by analysing the concentration time course of chemicals in the receptor fluid of diffusion cells and by determining the deposition in skin. Both skin cleaning procedures reduced the amount of fluoride in the skin compartments (p<0.05) and the receptor fluid (p<0.1). However, the effect of cleaning on the dermal absorption of the organic test compounds was not significant. The authors concluded that the results demonstrate the suitability of the applied ex vivo protocol for investigating the effectiveness of skin cleaning measures following dermal exposure. In addition, data reveal that the determination of test compounds in both, skin compartments as well as receptor fluid as equivalent for the systemic uptake needs to be considered in studies assessing the effectiveness of skin decontamination procedures. Authors: Dennerlein K, Jäger T, Göen T, Kilo S, Schaller KH, Drexler H, Korinth G. ;Full Source: Toxicology In Vitro. 2015 Mar 16;29(5):828-833. doi: 10.1016/j.tiv.2015.03.001. [fusion_builder_container hundred_percent="yes" overflow="visible"][fusion_builder_row][fusion_builder_column type="1_1" background_position="left top" background_color="" border_size="" border_color="" border_style="solid" spacing="yes" background_image="" background_repeat="no-repeat" padding="" margin_top="0px" margin_bottom="0px" class="" id="" animation_type="" animation_speed="0.3" animation_direction="left" hide_on_mobile="no" center_content="no" min_height="none"][Epub ahead of print] ;[/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]