The indiscriminate use of nerve agents by terrorist groups has attracted attention of the scientific communities toward the development of novel sensor technique for these deadly chemicals. A photonic crystal (PhC) hydrogel immobilised with butyrylcholinesterase (BuChE) was firstly prepared for the sensing of Sarin agents. Periodic polystyrene colloidal (240nm) array was embedded inside an acrylamide hydrogel, and then BuChE was immobilised inside the hydrogel matrix via condensation with 3-(diethoxyphosphoryloxy)-1,2,3-benzotriazin-4(3h)-one (DEPBT). It indicated that a total of 3.7 units of BuChE were immobilised onto the PhC hydrogel. The functionalised hydrogel recognised the Sarin agent and then shrunk, thus the diffraction of PhC hydrogel blue shifted significantly, and a limit of detection (LOD) of 10(-15) mol L (-1) was achieved.
Authors: Yan C, Qi F, Li S, Xu J, Liu C, Meng Z, Qiu L, Xue M, Lu W, Yan Z. ;Full Source: Talanta. 2016 Oct 1; 159:412-7. doi: 10.1016/j.talanta.2016.06.045. Epub 2016 Jun 21. ;