Gene expression analysis in rat lungs after intratracheal exposure to nanoparticles doped with cadmium

Silica nanoparticles (NPs) incorporating cadmium (Cd) have been developed for a range of potential application including drug delivery devices. Occupational Cd inhalation has been associated with emphysema, pulmonary fibrosis and lung tumours. Mechanistically, Cd can induce oxidative stress and mediate cell-signalling pathways that are involved in inflammation. This in vivo study aimed at investigating pulmonary molecular effects of NPs doped with Cd (NPCd,1 mg/animal) compared to a solution of CdCl2 (400 íg/animal), in Sprague Dawley rats treated intra-tracheally, 7 and 30 days after administration. NPs of silica containing Cd salt were prepared starting from nano-size silica powder (HiSil T700 Degussa) with average pore size of 20 nm and surface area of 240 m2/g. Toxicogenomic analysis was performed by the DNA microarray technology (using Agilent Whole Rat Genome Microarray 4 44K) to evaluate changes in gene expression of the entire genome. These findings indicate that the whole genome analysis may represent a valuable approach to assess the whole spectrum of biological responses to cadmium containing nanomaterials.

Authors: Coccini, Teresa; Fabbri, Marco; Roda, Elisa; Sacco, Maria Grazia; Manzo, Luigi; Gribaldo, Laura ;Full Source: Journal of Physics: Conference Series 2011, 304, 012025/1-012025/10 (Eng) ;