Genetic Diversity of Human Adenovirus in Children with Acute Gastroenteritis, Albania, 2013-2015

The objectives of the present study were to assess the occurrence of human adenoviruses (HAdVs) in paediatric patients with gastroenteritis in Albania and to characterise HAdV strains. Faecal specimens from children admitted with acute gastroenteritis to the Paediatric Hospital in Tirana were screened for HAdV, using broad-range primers targeting the hexon gene, in combination with species-specific primers targeting the fibre gene. Phylogenetic analysis was then performed to assess the genetic relationships among the different sequences and between the sequences of the samples and those of the prototype strains. Adenovirus DNA was detected in 33/142 samples (23.2%); 14 belonged to species F(13 HAdV-41 and 1 HAdV-40), 13 to species C (1 HAdV-1, 8 HAdV-2, and 4 HAdV-5), 5 to species B (HAdV-3), and 1 to species A (HAdV-12). Rotavirus co-infection was present in 9/33 (27.2%) positive samples. In the remaining 24 positive samples (12 enteric-F species; 12 nonenteric-A, B, or C species), HAdVs were detected as unique viral pathogens, suggesting that HAdV may be an important cause of diarrhoea in children requiring hospitalisation. This is the first study investigating the presence of human adenoviruses (species A-G) as etiologic agents of viral gastroenteritis in children in Albania.

Authors: La Rosa G, Della Libera S, Petricca S, Iaconelli M, Donia D, Saccucci P, Cenko F, Xhelilaj G, Divizia M. ;Full Source: BioMed Research International. 2015;2015:142912. doi: 10.1155/2015/142912. Epub 2015 Aug 3. ;