High butter-fat diet and bisphenol A additively impair male rat spermatogenesis

Exposure to xenoestrogens is a probable cause of male infertility in humans. Consumption of high-fat diets and exposure to bisphenol A (BPA) is pervasive in America. In the present study, the authors tested the hypothesis that gestational exposure to high dietary fats and/or BPA disrupt spermatogenesis in adulthood. Sprague-Dawley rats were fed diets containing 10 kcal% butter fat (AIN), 39 kcal% butter fat (HFB), or 39 kcal% olive oil (HFO), with or without BPA (25?g/kg body weight/day) during pregnancy. One group of male offspring received testosterone (T)- and oestradiol-17? (E2)-filled implants or sham-implants from postnatal day (PND)70-210. Another group was naturally aged to 18 months. The authors discovered that adult males with gestational exposure to BPA, HFB, or HFB+BPA, in both the aged group and the T+E2-implanted group, exhibited impairment of spermatogenesis. In contrast, gestational exposure to HFO or HFO+BPA did not affect spermatogenesis. Sham-implanted, gestational exposed groups also had normal spermatogenesis. Loss of ER? expression in round spermatids and premature expression of protamine-1 in diplotene spermatocytes were features associated with impaired spermatogenesis. Compared with the single-treatment groups, the HFB+BPA group experienced more severe effects, including atrophy.

Authors: Tarapore P, Hennessy M, Song D, Ying J, Ouyang B, Govindarajah V, Leung YK, Ho SM. ;Full Source: Reproductive Toxicology. 2016 Sep 19. pii: S0890-6238(16)30339-2. doi: 10.1016/j.reprotox.2016.09.008. [Epub ahead of print] ;