Identification and Quantification of 1-Hydroxybutene-2-yl Mercapturic Acid in Human Urine by UPLCHILIC-MS/MS as a Novel Biomarker for 1,3-Butadiene Exposure

1,3-Butadiene (BD) is a Class 1 carcinogen present in polluted air of workplaces, in automobile exhaust, and in tobacco smoke. 2-Hydroxybutene-1-yl mercapturic acid (2-MHBMA) is a urinary metabolite often measured as a biomarker for exposure to BD. During this study, the authors demonstrated for the first that an additional MHBMA isomer is present at significant amounts in human urine, 1-hydroxybutene-2-yl mercapturic acid (1-MHBMA). For its quantification, a highly sensitive UPLC-HILIC-MS/MS method was developed and validated. Analysing urinary samples of 183 volunteers, the authors demonstrate that 1-MHBMA is a novel and potentially more reliable biomarker for BD exposure than the commonly analysed 2-MHBMA.

Authors: Sterz, Katharina; Scherer, Gerhard; Krumsiek, Jan; Theis, Fabian J.; Ecker, Josef ;Full Source: Chemical Research in Toxicology [fusion_builder_container hundred_percent=”yes” overflow=”visible”][fusion_builder_row][fusion_builder_column type=”1_1″ background_position=”left top” background_color=”” border_size=”” border_color=”” border_style=”solid” spacing=”yes” background_image=”” background_repeat=”no-repeat” padding=”” margin_top=”0px” margin_bottom=”0px” class=”” id=”” animation_type=”” animation_speed=”0.3″ animation_direction=”left” hide_on_mobile=”no” center_content=”no” min_height=”none”][online computer file] 2012, 25(8), 1565-1567 (Eng) ;