In vitro evaluation of the genotoxicity of ritodrine and verapamil in human lymphocytes

The aim of this study was to investigate the genotoxic effects of ritodrine and verapamil on human peripheral lymphocytes in vitro using micronucleus (MN) test. In addition, fluorescence in situ hybridisation (FISH) with a centromeric probe was performed to detect the origin of the induced MN. Cells were treated with 8.4 x 10-6 M – 25.2 x 10-4m concentrations for ritodrine and 0.56-11 10-5 M concentrations for verapamil, separated and combined. The MN frequencies showed increase after all treatments, but the difference between treated cells and untreated controls were found to be statistically significant only in the concentration range from 8.4 x 10-5 M -4.5 x 10-4 M for ritodrine, 1.1 – 3.3 x 10-5 M for verapamil, and in combined treatment with concentrations. 8.4 x 10-5 M + 1.1 x 10-5 M for ritodrine and verapamil. The highest tested concentration of both medicaments showed cytotoxic effect. Both medicaments decreased the nuclear division index (NDI) in tested concentrations. The results of FISH analysis suggest that verapamil, separate or combined with ritodrine, shows to a larger extent aneugenic than clastogenic effect.

Authors: Milosevic-Djordjevic, O.; Grujicic, D.; Joksic, G.; Marinkovic, D. ;Full Source: Human & Experimental Toxicology 2011, 30(5), 398-405 (Eng) ;