Increase of micronuclei frequencies of peripheral blood lymphocyte in plywood workers exposed accumulatively to formaldehyde

In this study, the effect of occupational exposure to formaldehyde on micronuclei frequency in peripheral blood lymphocytes of workers was investigated. Two hundred thirty six plywood workers were divided into 3 exposure groups (low, middle and high) according to internal exposure biomarker (formaldehyde human serum albumin conjugate, FA-HSA), which was detected by ELISA. The concentrations of formaldehyde (FA) in the air of two workshops were measured using high performance liquid chromatography. Cytokinesis-block micronucleus (CBMN) test was used to detect the micronuclei frequency of peripheral blood lymphocyte in 236 workers. The results demonstrated that the average concentrations of FA in the low and high exposure workshops were 0.58(0.20 and 1.48(0.61 mg/m3, respectively, and there was significant difference (P<0.01). The average concentrations of serum FAHAS of workers in the two workshops were 69.22(15.37 and 136.29(89.49 pg/mL respectively, and there was significant difference (P<0.01). The results of CBMN test showed that the micronucleus frequencies in the low, middle and high exposure groups were 1.94(1.72, 2.10(1.92 and 2.10(1.70%, respectively, and there were no significant differences between groups. However, the micronucleus frequencies in the accumulative low, middle and high exposure groups were 1.36(1.36, 2.31(1.81 and 2.49(1.92%, respectively, and there were significant differences between different accumulative exposure groups (P<0.0l). The results of correlation analysis indicated that there was a positive correlation between accumulative exposure levels and micronucleus frequencies (rs)0.321, P<0.01). The accumulative exposure doses may be a risk factor for high micronucleus frequencies in workers exposed to FA (Ptrend)0.002). The authors concluded that based on the results for the present study, FA-HSA levels can serve as an internal exposure biomarker for assessing the exposure level of workers exposed to FA. Accumulative formaldehyde exposure resulted in an increase of micronuclei frequencies of peripheral blood lymphocyte in plywood workers.

Authors: Guo, Yaojing; Lin, Dafeng; Yi, Jihu; Kuang, Dan; Deng, Huaxin; Li, Xiaohai; Zhang, Zhihu; Wu, Tangchun ;Full Source: Zhonghua Laodong Weisheng Zhiyebing Zazhi 2012, 30(1), 17-20 (Chinese) ;