In this study the effect of silver nanoparticles (AgNPs) on proliferation of oestrogen receptor (ER)-positive human breast cancer MCF-7/BUS cells were assessed by means of in vitro assay. The cells were exposed in the absence of oestrogens to AgNPs alone or in combination with aluminium chloride (AlCl3), butyl paraben (BPB) and di-n-butyl phthalate (DBPh). The results revealed that AgNPs at the non-cytotoxic concentrations (up to 2?g/mL) and AlCl3 (up to 500?M) did not induce proliferation of MCF-7/BUS cells whereas BPB and DBPh showed strong oestrogenic activity with the highest effect at 16?M and 35?M, respectively. AgNPs inhibited the proliferation of the cells induced by DBPh, BPB or even with 17?-oestradiol (E2) during 6-day incubation in the absence of oestrogens. ICI 182,780 (10nM), a known oestrogen receptor (ER) antagonist, induced strong inhibitory effect. AgNPs also decreased transcription of the oestrogen-responsive pS2 and progesterone receptor (PGR) genes but modulated expression neither of ER? nor ER? in MCF-7/BUS cells exposed to BPB, DBPh or E2 for 6h. These results indicate that AgNPs may inhibit growth of breast cancer cells stimulated by E2 or oestrogenic chemicals, i.e. BPB and DBPh.
Authors: Roszak J, Smok-Pieni??ek A, Domeradzka-Gajda K, Grobelny J, Tomaszewska E, Ranoszek-Soliwoda K, Celichowski G, St?pnik M. ; Full Source: Toxicology & Applied Pharmacology. 2017 Oct 23; 337:12-21. doi: 10.1016/j.taap.2017.10.014. [Epub ahead of print]