Lead acetate induces cell apoptosis and promotes caspase-3 expression in human liver cell line L-02

The impact of lead acetate on cell apoptosis and caspase-3 expression in human liver cell line L-02 was investigated. L-02 cells were treated with different concentrations (0, 2.5, 40, 100, 200, 400 ímol/L) of lead acetate for 24 or 48 h. Compared to the normal control group, the proliferation of L-02 cells was significantly inhibited after treatment with different concentrations of lead acetate for 24 or 48 h. Significant morphological changes were noted in cells treated with lead acetate for 48 h. Compared to the normal control group, the levels of caspase-3 mRNA increased significantly in a dose-dependent manner in L-02 cells treated with lead acetate. Additional, the expression levels of caspase-3 protein also significantly increased after treatment with lead acetate. Lead acetate inhibits proliferation and induces apoptosis of L-02 cells possibly via a mechanism associated with the activation of caspase-3.

Authors: Xing, Wei; Li, Shenglian; Chen, Zhaoyi ;Full Source: Shijie Huaren Xiaohua Zazhi 2011, 19(23), 2426-2431 (China) ;