Measurements of polybrominated diphenyl ethers and polychlorinated biphenyls in a single drop of blood

A quantity that requires only a small volume (50 íL) of blood has been developed for the detection of polybrominated di-Ph ethers (PBDEs) and polychlorinated biphenyls (PCBs). Target analytes in both plasma sample (DBSV) and dried blood spot (DBS) were analysed by a gas chromatography/high resolution mass spectrometer (GC/HRMS). Measurements of standard reference by the developed method were in agreement with those certified values. Linear correlation coefficients were found to be 0.9984 and 0.9965 for DBS and DBSV analysis, respectively. Other analysis criteria, such as limits of detection, recoveries, precision, accuracy and linearity of the proposed method are also reported. From recovery studies, the addition of formic acid to the extension solvent was found to be effective in extending PBDEs and PCBs from filter paper. The PBDE and PCB levels in spiked DBS were monitored at room temperature for up to 30 days and the variations of target analytes were found to be insignificant. The authors’ results suggest that DBS sampling technique is feasible for PBDE and PCBs biomonitoring in human population.

Authors: Lu, Dasheng; Wang, Dongli; Ip, Ho Sai Simon; Barley, Frank; Ramage, Robert; She, Jianwen. ;Full Source: Journal of Chromatography, B: Analytical Technologies in the Biomedical and Life Sciences [online computer file] 2012, 891-892, 36-43 (Eng) ;