Nano-silicon dioxide toxicological characterisation on two human kidney cell lines

Silicon dioxide nano-particles (n-SiO2) have recently encountered a wide variety of applications in medicine or engineering but their toxicology effects are poorly understood. In this study, SiO2-25 nm and SiO2-100 nm mono-dispersed nano-particles were used and labelled with Rhodamine B and TMPyP respectively. These two fluorophores were incorporated during synthesis in order to track nano-particles cell incorporation. Upto-date, no evaluation of the toxicology effects of these nano-particles upon human kidney has been published. The aim of this study is to evaluate the potential toxicity of these nano-particles on two human cell lines from proximal tubule (Caki-1 and Hek293). The results report that the two cell lines do not show similar responses after 24 h of exposure to SiO2-nanoparticles disregarding a similar origin in the kidney. These results indicate that for both tested SiO2-nano-particles, caki-1 cells present a higher sensitivity in terms of cytotoxicity and genotoxicity than Hek293 cells. Also, the results show that for similar concentration of exposure, SiO2-25 nm seems to be more cytotoxic and genotoxic than SiO2-100nm for both tested cell lines.

Authors: Paget, V.; Sergent, J. A.; Chevillard, S. ;Full Source: Journal of Physics: Conference Series [online computer file] 2011, 304, 012080/1-012080/10 (Eng) ;