Optimising transfection of human umbilical cord-derived mesenchymal stem cells using novel chemical transfection reagents

Genetic modification technology of human umbilical cord-derived mesenchymal stem cells (hUCMSCs) is numerous and varies in the complexity of their efficiency, reliability and safety. Transfected efficiency of different novel chemical transfection reagents on hUCMSCs was compared. The hUCMSCs were transfected with Fugene HD, Lipofectamine LTX, and Attractene Poison cells were detected under fluorescent microscope, and expression efficiencies were measured by using flow cytometry. Cell viability was measured by using trypan blue exclusion after transfection. The highest transfected efficiency was observed with Lipofectamine LTX (32.5(2.12)% (P<0.05), followed by Fugene HD (4.3(0.64)%, minimal transfection efficiency was obsessed with Attractene (1.74(0.08)%. Lipofectamine LTX demonstrated the lower cell viability (69.8(6.3)% compared with Fugene HD (92.4(4.2)% and Attractene (106.6(3.9)% (P>0.05), but the difference was not significant. In summary, this study demonstrates that Lipofectamine LTX is a relative ideal chemical transfection reagent for hUCMSCs.

Authors: Lu, Jun; Zhao, Hongzhou; Chen, Jin; Fu, Yunfeng; Huang, Lianghu; Tan, Jianming ;Full Source: Zhongguo Zuzhi Gongcheng Yanjiu Yu Linchuang Kangfu 2011, 15(49), 9151-9154 (Ch) ;