In this study, the authors describe the SERS and phospholipid-ganglioside GM1 membrane-coated gold nano-particle probe for detecting cholera toxin in food or water sample. The title method for detecting cholera toxin comprises the steps of: (1) preparing Bimolecule phospholipid-ganglioside GM1 membrane coated gold nanoparticle probes; 2) specifically combining cholera toxin B subunit with multiple receptor GM1 molecules in the bimolecular phospholipid membrane to obtain aggregated bimolecular phospholipid membrane coated gold nanoparticle probes; and 3) detecting – The bimolecular phospholipid membrane comprises ganglioside GM1 molecules and hydrosulphide group-modified phospholipid molecules. This method can be used for biological sensing cholera toxin by Surface-Enhanced Resonance Raman Spectrum analysis (SERS) and UV-visible light absorption spectroscopy or colorimetry. The method has the advantages of simple operation, rapid detection, no labelling process, low cost, high sensitivity and high specificity. The invention can be used for food safety monitoring and preventing food induced diseases.
Authors: Wang, Yu; Jiang, Jianhui; Chu, Xia; Tang, Lijuan; Yu, Ruqin ;Full Source: Application 10,116,434, 19 April 2012; 6pp. (Chinese) ;