Contaminant exposure in aqueous systems typically involve complex chemical mixtures. Given the large number of compounds present in the environment, it is critical to rapidly identify hazardous chemical interactions. This study utilises a prototype for a novel high throughput assay to quantify behavioural changes over time to identify chemical interactions that affect toxicity. The independent and combined effects of two chemicals, diazinon (an insecticide) and 4-nonylphenol (a detergent metabolite) on the swimming behaviour of the freshwater crustacean, Daphnia pulex were examined. Cumulative distance and change in direction were measured repeatedly via optical tracking over 90-minutes. Exposure to low concentrations of diazinon (0.125 to 2 µM) or 4-nonylphenol (0.25 to 4µM) elicited significant concentration- and time-dependent effects on swimming behaviour. Exposure to 0.5 µM 4-nonylphenol alone did not significantly alter mean cumulative distance, but did elicit a small, significant increase in mean angle, the measure of change in direction. When 0.5 µM 4-nonylphenol was used in combination with diazinon (0.125 to 0.5 µM) it augmented the adverse impact of diazinon on the swimming behaviour of Daphnia. Additionally, enhanced sensitivity to diazinon was observed in animals exposed to treated wastewater effluent for 24 hours prior to a diazinon challenge. The authors concluded that these experiments demonstrate that exposure to 4-nonylphenol and complex chemical mixtures (e.g. treated wastewater), can enhance the toxicity of exposure to the insecticide diazinon.
Authors: Zein MA, McElmurry SP, Kashian DR, Savolainen PT, Pitts DK. ;Full Source: Environmental Toxicology & Chemistry. 2015 Feb 5. doi: 10.1002/etc.2908. [fusion_builder_container hundred_percent=”yes” overflow=”visible”][fusion_builder_row][fusion_builder_column type=”1_1″ background_position=”left top” background_color=”” border_size=”” border_color=”” border_style=”solid” spacing=”yes” background_image=”” background_repeat=”no-repeat” padding=”” margin_top=”0px” margin_bottom=”0px” class=”” id=”” animation_type=”” animation_speed=”0.3″ animation_direction=”left” hide_on_mobile=”no” center_content=”no” min_height=”none”][Epub ahead of print] ;[/fusion_builder_column][/fusion_builder_row][/fusion_builder_container]