Transcriptional profiling of the soil invertebrate Folsomia candida in pentachlorophenol-contaminated soil

Pentachlorophenol (PCP), a widely used pesticide, is considered to be an endocrine disruptor. The molecular effects of chemicals with endocrine-disrupting potential on soil invertebrates are largely unknown. In the present study, the authors explored the transcriptional expression changes of collembola (Folsomia candida) in response to PCP contamination. A total of 92 genes were significantly differentially expressed at all exposure times, and the majority of them were found to be down regulated. In addition to the transcripts encoding cytochrome P450s and transferase enzymes, chitin-binding protein was also identified in the list of common differentially expressed genes. Analyses of gene ontology annotation and enrichment revealed that cell cycle-related transcripts were significantly induced by PCP, indicating that PCP can stimulate cell proliferation in springtail, as has been reported in human breast cancer cells. Enrichment of functional terms related to steroid receptors was observed, particularly in 20 significant differentially expressed genes involved in chitin metabolism in response to PCP exposure. Combined with confirmation by quantitative polymerase chain reaction, the results indicate that the adverse effects on reproduction of springtails after exposure to PCP can be attributed to a chemical-induced delay in the moulting cycle and that moulting-associated genes may serve as possible biomarkers for assessing toxicological effects.

Authors: Qiao M, Wang GP, Zhang C, Roelofs D, van Straalen NM, Zhu YG. ;Full Source: Environmental Toxicology & Chemistry. 2015 Jun;34(6):1362-8. doi: 10.1002/etc.2930. Epub 2015 Apr 13. ;