Polycyclic aromatic hydrocarbons (PAHs) are ubiquitously found in the environment and have been proved to be prospectively associated with the risk of cancer. In this study, a simple method based on pipette-tip solid phase extraction (PT-SPE) and gas chromatography-mass spectrometry (GC-MS) has been firstly developed for the determination of 16 PAHs in human whole blood. Three-dimensional ionic liquid-ferrite functionalised graphene oxide nanocomposite (3D-IL-Fe3O4-GO) was used as sorbent in PT-SPE. Compared with conventional SPE method, the PT-SPE method was solvent-saving (1.0?mL), reusable (at least 10 times) and required less blood sample (200??L). Affecting parameters on extraction efficiency were investigated and optimised. Under the optimised conditions, a good linearity was obtained and the recoveries of 16 PAHs at three spiked levels ranged from 85.0% to 115%. The limits of quantification (LOQs) were in the range of 0.007-0.013??g/L. Furthermore, the developed method was successfully applied to the analysis of 16 PAHs in 14 human blood samples. The results showed that the predominant PAHs in human whole blood was low-molecular-weight PAHs, with the rank order phenanthrene (PHE)> naphthalene (NAP)> fluorene (FLU)> fluoranthene (FLT)> pyrene (PYR). Because of its simplicity, accuracy and reliability, the PT-SPE method combined with GC-MS demonstrated the applicability for clinical analysis and provided more information for PAHs exposure studies.
Authors: Zhang Y, Zhao YG, Chen WS, Cheng HL, Zeng XQ, Zhu Y. ; Full Source: Journal of Chromatography A. 2018 Jun 1; 1552:1-9. doi: 10.1016/j.chroma.2018.03.039. Epub 2018 Mar 21.